1-[[6-(2,5-Dihydro-2,5-dioxo-1H-pyrrol-1-yl)-1-oxohexyl]oxy]-2,5-dioxo-3-pyrrolidinesulfonic Acid SodiuM Salt - Names and Identifiers
1-[[6-(2,5-Dihydro-2,5-dioxo-1H-pyrrol-1-yl)-1-oxohexyl]oxy]-2,5-dioxo-3-pyrrolidinesulfonic Acid SodiuM Salt - Physico-chemical Properties
Molecular Formula | C14H16N2O9S
|
Molar Mass | 410.33 |
Melting Point | 158-165°C |
Solubility | DMSO (Slightly, Sonicated) |
Appearance | Solid |
Color | White to Pale Yellow |
Storage Condition | 2-8°C |
Stability | Moisture Sensitive |
1-[[6-(2,5-Dihydro-2,5-dioxo-1H-pyrrol-1-yl)-1-oxohexyl]oxy]-2,5-dioxo-3-pyrrolidinesulfonic Acid SodiuM Salt - Introduction
ε-maleimide hexanoate sulfosuccinimide ester (Sulfo-EMCS) is a compound with the following properties:
1. Appearance: White to off-white solid.
2. molecular formula: C15H16N2O10S2.
3. Molecular weight: 460.42g/mol.
4. solubility: soluble in water.
Sulfo-EMCS is mainly used for covalent coupling of proteins and functional molecules. As an active development reagent, it can react with biological macromolecules (such as proteins or peptides) containing sulfhydryl groups (-SH) to form stable covalent linkages. This makes Sulfo-EMCS widely used in biochemical research to construct the connection between protein or peptide and other molecules, such as fluorescent labeling, enzyme coupling and immunoprecipitation.
The preparation Sulfo-EMCS is usually carried out by the following steps:
1. reacting ε-maleimidocaproic acid chloride with succinimidyl ester.
2. The reaction is carried out in an appropriate solvent.
3. Purification of the product, for example by solvent extraction and crystallization.
It should be noted that Sulfo-EMCS is a chemical reagent and must be used under appropriate conditions and personal protective equipment. In addition, it should also be stored in a dry and cool place to avoid prolonged exposure to light and high temperatures. Complete safety information should be obtained from the supplier. In the process of use, should strictly abide by the safety procedures, avoid contact with skin, eyes and respiratory tract. If inadvertently exposed to the compound, rinse immediately with plenty of water and seek medical help.
Last Update:2024-04-10 22:29:15